The effect of nano Silicon on BAX expression of liver and kidney in rats

Today the application of nanotechnology in the various stages of food production, processing and packaging has gained special prominence.Nano silicon is a nanoparticle used as an antibacterial food additive in animal feed, poultry and in food packaging.Given the potential of this nanomaterial to enter the human food chain, it is important to evaluate its risk for its side effects and toxicity. The purpose of this study was to investigate the effect of nano silicon on one of the apoptotic marker. For this purpose, ۲۰ female rats were separated into ۴ groups of ۵ each and received ۳۰۰, ۶۰۰ and ۹۰۰ mg/kg/day of silicon nanoparticles at ۲۰-۳۰ nm size orally. On the ۲۰th day the rats were euthanized and their liver and kidneys were removed and transferred to a temperature at -۸۰ C°.The cDNA synthesized using specific primers was used to amplify the GLUT-۲ gene and take into account a housekeeping gene in real-time PCR.Expression of Bax protein in kidney and liver was evaluated by Real Time - PCR. Relative expression of Bax gene in kidney and liver between different groups showed that Bax gene expression in liver was increased in groups receiving ۳۰۰ mg and ۹۰۰ mg nano silicon.

Keywords:

apoptosis , BAX , Real Time-PCR.