Study of structure and comparison of TRBF protein sequences in plants, using bioinformatics and computational methods

Background and Aim: There is a balance between decreasing and increasing telomere length in cells. That is, certain molecular systems with a gradual decrease in telomere length and reaching a certain limit allows for its length to be increased. Telomere Binding Proteins based on the binding sites, on the telomeric regions, as well as the presence of these proteins Increases and decreases telomerase activity. And as a result, telomere length is synthesized. The evolution of telomere-binding proteins and their structure in different species is controversial. The aim of this study was to collect and compare TRBF (Repeat Binding Factor) gene family sequences to find conserved motifs and to investigate the affinities of this gene, it was different between plants. This is done using bioinformatics tools. In this work, for the first time, studies have been carried out on ۳ levels of protein, mRNA and DNA. As the sequence of telomeric region sequences of organisms is constantly increasing and decreasing, information of these regulatory proteins and synthesis of the above telomeric region in different plants has been considered.Methods: Species were selected with at least ۴۵% evolutionary similarity in whole plants. Sequences were aligned with the ClastalW program. The evolutionary tree of organisms was plotted with the Mega۵ program. And the Scanprosite program was used to search for the protein motif. The protein structure was also drawn by the ExPASy program.Results: TRBF affinities between different plant species were reported in the N-terminal and C-terminal segments. Specific conserved amino acid fragments and the second reverse transcriptase were introduced as the functional conserved motif of all species at the protein level.Conclusion: Deletion or disruption of the PTB domain, will result in inefficiency of the TRBF protein in all species. Also, central motifs similar to this protein among species can be a suitable target for the expression or non-expression of TRBF proteins, which consequently increases and decreases telomerase activity in telomeric regions.

Keywords:

TRBF protein , Telomeric regions , Domain