ناشر تخصصی کنفرانس های ایران

لطفا کمی صبر نمایید

Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها
Paper
Title

The effect of electrospun zein nanofiber coated with Nano hydroxyapatite on osteogenic differentiation of mesenchymal stem cells

Year: 1399
COI: CIGS16_452
Language: EnglishView: 152
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

Roksana Raefi - student
zivar salehi - Supervisor
mahvash hadavi - Advisor

Abstract:

Background and Aim: Tissue engineering requires comprehensive efforts to combine engineering and physical sciences with life sciences for the purpose of restoring, replacing, or improving the function of damaged tissues and organs. This method is based on three main factors: a scaffold, cells and growth factors. The scaffold, a three-dimensional construct, is designed to support cell infiltration, growth, differentiation, and enhance new tissue development and guide new tissue formation. Among various classes of biomaterials, polymer-based scaffolds are the primary choice for almost all tissues. zein, is a natural polymer isolated from corn which recently regarded as a promising biopolymer for tissue engineering scaffold on account of its amphiphilic nature, nontoxicity, favorable biodegradability and bio-compatibility but like all natural polymers, zein has poor mechanical properties. In order to improve their mechanical properties and water resistance, plant proteins can be blended with inorganic compounds like nano Hydroxyapatite (nHA) that is the main inorganic component of bone.Methods: Zein/nHA nanofibrous structures were fabricated by the electrospinning method. A polymer solution was prepared by dissolving zein in acetic acid. Zein nanofibers coated with Nano hydroxyapatite after fabrication. Surface morphology and pore size of nanocomposite scaffolds were analyzed by images of scanning electron microscope (SEM). Average fiber diameters and Surface pore sizes of nanofibrous scaffolds were determined using ImageJ software. The chemical structure characterized by Fourier transform infrared spectroscopy (FTIR). For invitro assays, the scaffolds cut into small pieces (۱×۱ Cm۲) and sterilized with UV and antibiotics before cells seeding. Human Mesenchymal stem cell was seeded with density of ۵×۱۰۴ cells on Zein Scaffolds. MTT assay was used for cell proliferation assessment after ۴۸h and total RNA was extracted for Osterix and β-catenin genes expression levels in different time period.Results: The diameter of nanofibers was estimated between ۳۳۵-۸۲۸ nm and ۲۹۶-۷۲۲ in nHA coated and control mate respectively. The FTIR spectrum of bulk zein and nanofiber scaffolds had no difference in peaks so no new chemical interaction was suggested and nHA associated to zein nanofibers with physical force. Zein nanofibers in both group had appropriate cell compatibility. The Osterix and β-catenin genes expression elevated significantly in nHA coted zein nanofibers respect with control and zein nanofibers. Conclusion: The electrospun zein nanofiber coated with Nano hydroxyapatite had good effect on osteogenic differentiation of mesenchymal stem cells and may use in bone tissue engineering.

Keywords:

tissue engineering, zein, Nano hydroxyapatite, osteogenic differentiation

Paper COI Code

This Paper COI Code is CIGS16_452. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1195718/

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Raefi, Roksana and salehi, zivar and hadavi, mahvash,1399,The effect of electrospun zein nanofiber coated with Nano hydroxyapatite on osteogenic differentiation of mesenchymal stem cells,the fourth International and 16th National Genetics Congress,Tehran,https://civilica.com/doc/1195718

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.

New Papers

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.

Support