TERT Promoter Polymorphisms and Risk of Cervical Cancer

TERT It has been shown that TERT is overexpressed in ۹۰% of human cancers, and genetic alterations in the proximal promoter of TERT are significantly associated with a variety of different cancer types. In recent years, a new mechanism of TERT regulation through the non-coding driver mutations (C۲۲۸T and C۲۵۰T) in the TERT promoter has been reported in several cancer types. In the present study, we investigated the relationship between the Single Nucleotide Polymorphism (SNP) rs۲۸۵۳۶۶۹ and cervical cancer. The study included ۸۰ individuals, including ۵۰ patients with cervical cancer and ۳۰ healthy controls. The samples from the participants underwent sequencing and genotyping using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method. It was found that ۱۶%, ۲۴%, and ۶۰% of the cervical cancer samples had the genotypes of AA, AG, and GG, respectively. In the control group, the frequencies were ۱۳.۳۳%, ۵۰%, and ۳۶.۶۶% of the samples for the genotypes of AA, AG, and GG, respectively.  According to our findings, there was a significant association between the recessive model GG vs. AA+AG and cervical cancer susceptibility.  

Keywords:

cervical cancer , Telomerase , TERT , PCR-RFLP