Prevalence of racR gene in thermophilic Campylobacter jejuni andCampylobacter coli isolated in poultry

Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans.The purpose of this study was determined the prevalence ofrecR gene in Campylobacter jejuni and Campylobacter coli isolated from poultry. To perform the study ۱۹۶fecal samples were collected from poultry. All samples were subjected for isolation of Campylobacter spp.using prêt KB method and the presumptive isolates authenticated by DNA sequencing of ۱۶srRNA genes.Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that ۳۹strains of Campylobacter spp. were isolated. Of all isolates ۳۰ strains were identified as Campylobacter jejuniand the rest (۹ isolates) was Campylobacter coli. The genome of ۲۴ (۸۰%) C.jejuni and ۹ (۱۰۰%) C.coli werecontained racR gene. Hence based on foregoing evidence racR gene existed approximately in all isolates.Therefore, Campylobacter strains isolated from different sources could be considered infectious agentfor human. It is because phenotypical character inducted by racR gene (colonization and heat tolerance) helpsthem to survive and cause infection.

Keywords:

racR gene , Thermophilic Campylobacters , Poultry