Study the antimicrobial effects of Thyme, Aloe vera and Zararia multiflora extracts against antibiotics resistant Pseudomonas aeruginosa

Background and aims: Resistance of bacteria against antibiotics has always been a prevalent problem in medicine. So, finding new antimicrobial compounds with least side effects seems to be necessary. This paper aims to explore the antimicrobial effects of Thyme, and Aloe vera and Zararia multiflora extracts against Pseudomonas aeruginosa resistant to antibiotics.Methods: Thyme, and Aloe vera and Z. Multiflora extracts were obtained by using rotary devices. Twenty samples of P. aeruginosa were collected from the patients being cured in Zabol hospitals. The least hindering and killing concentrations of the samples were calculated by lowering their density in sinks. Sensitivity of P. aeruginosa to the different antibiotics prepared by Padtan Teb Co was evaluated by the diffusion disk standard method proposed by Kirby Bauer. Data were analyzed statistically by determination of significant difference using analysis of variance (ANOVA) test. All tests were analyzed at the significance level P<۰.۰۵.Results: Our results have shown that Aloe vera hinders the growth of bacteria in various concentrations. Despite the relative resistance of most of the samples in the used concentrations, the highest sensitivity was observed in ۱۰ and ۲۰ mg/l. Approximately, Aloe vera extract has shown the highest hindrance effect in ۵ mg/l. Further, the highest sensitivity to Z. Multiflora was in ۱۰ and ۲۰ mg/l in which ۱۰۰% of the bacteria were killed. Approximately, Z. Multiflora of ۵mg/l had the highest resistive effect.Conclusion: Thyme, and Aloe vera and Z. Multiflora extracts have considerable antimicrobial effect on the samples of the P. aeruginosa resistant to antibiotics.

Keywords:

Antimicrobial effects , Antibiotic resistant , Pathogens , Plant extract