Effects of fennel, asafetida and ginseng ethanolic extracts on growth and proliferation of mouse breast cancer ۴T۱ cell lines

Background: The ۴T۱ cells tumor growth and metastatic pattern in BALB/c mice very closely mimic human breast cancer. These herbal remedies used in traditional folk medicine have been the source of many medically beneficial drugs. The aim of the current study was to evaluate the anti-proliferative activity of the asafoetida, ginseng and fennel ethanolic extracts on mouse breast cancer ۴T۱ cell-line invitro.Method: in this experimental study, Asafoetida, gensing and fennel were extracted; ۴T۱ mouse mammary tumor cell line were cultured in ۴۸-well flat bottom plate in density of ۵۰x۱۰۳ per well in ۱۰۰ µl RPMI۱۶۴۰ medium then different dilutions of each extract (۲۵ , ۵۰, ۱۰۰, ۲۰۰, ۵۰۰, and ۲۰۰۰ μg/ml) were added to cell culture. Cells then were incubated at ۳۷ oC for ۲۴ hours. After ۲۴ h, cell Proliferation was determined by the BRDU assay . dataset of experiments were collected and analysed with SPSS۱۹ software. Significant level of Data was Examined with one-way ANOVA method and P

Keywords:

fennel , Asafetida , Ginseng , ۴T۱ Cell line , Breast cancer