Anticancer Screening of Various Seed Extract of Cardiospermum halicacabum on Human Colorectal, Skinand Breast Cancer Cell Lines

Background: In the modern lifestyle, the increase in cancer and related chronicdisorders is a major public health problem. In spite of different methods used forthe treatment of these conditions, natural medicines have high demands due totheir significant effects as immune enhancement and therapeutic agents and fewerside effects in comparison with other treatment methods. Hence, this study wasundertaken to evaluate the cytotoxic effect of cardiospermum halicacabum Linn.seeds, based on traditional claims.Methods:ASoxhlet extractor was used to obtain different extracts from seedsof C. halicacabum Linn. Sulforhodamine B colorimetric (SRB) assay used for theevaluation of the cytotoxic effect of the various extracts on HT-29, HCT-15 coloncarcinoma, SK-MEL-2 skin carcinoma, andMCF-7 breast carcinoma.The resultswere compared against Doxorubicin as a standard drug.Results: The results of the present study showed the potent cytotoxic activity ofn-hexane extract of seeds of C. halicacabum Linn. against the MCF-7 breastcancer cell line with 50% growth inhibition value (GI50) of 12.8 μg/ml but otherextracts showed poor activity in other tested cell lines.Conclusions: The results indicated the potential medicinal value of C.halicacabum Linn. seeds oil with the highest extractive yield as an antineoplasticagent. However, further studies are needed for the isolation of the active anticancercompounds and evaluating the mechanism of action of the responsible compound.

Anticancer Screening of Various Seed Extract of Cardiospermum halicacabum on Human Colorectal, Skinand Breast Cancer Cell Lines Keywords:

Skin cancer cytotoxicity , breast cancer , colon cancer , cardiospermum halicacabum