Effects of Camel Milk on Antioxidant Activity in Rats with Valproic Acid-induced Autism

Introduction: Autism spectrum disorder refers to a wide range of nervous system disorders. Autistic patients often have a defective antioxidant defense system and manifest symptoms of impaired cognitive interaction. Camel milk has numerous beneficial nutrients and has been used in the treatment of autism. The present study aimed to investigate the effects of camel milk on the antioxidant activity and enzymes of autistic rats. Methods: Pregnant rats were intraperitoneally injected on embryonic day ۱۲.۵ with valproic acid (VPA; ۵۰۰ mg/kg) to induce an autistic state. In addition, ۱۸ male offspring rats were injected with risperidone (۰.۲ mg/kg) three times per week. Six of these cases were fed daily with raw camel milk (۱۰ ml/kg), and six others were fed with pasteurized camel milk for ۴۲ days. Social interaction and repetitive behaviors were measured using the Y-maze based on catalase (CAT) activity, glutathione (GSH) level, and superoxide dismutase (SOD) activity at the outset and after the treatment period. Results: Behavioral symptoms (impaired social interaction and repetitive behaviors) were evident after VPA administration. After receiving treatment, impaired social interaction and repetitive behaviors significantly improved in the autistic rats (P<۰.۰۱). In addition, VPA enhanced the oxidative stress status in the biochemical tests (P<۰.۰۰۱), and camel milk increased CAT activity (P<۰.۰۰۱), GSH level (P<۰.۰۵), and SOD activity (P<۰.۰۰۰۱). Conclusion: According to the results, camel milk could recover the VPA-induced impairment of social interaction and repetitive behaviors in the autistic rats and improve the defects in their antioxidant defense system.

Keywords:

Antioxidant enzyme , Autism , Behavior test , VPA