A green nano-dressing with potent antibacterial effect

Background and Aim : Skin is the body's first defense line against environmental pathogens. However, open skin wounds can interfere with the normal function of the skin and the entry of opportunistic bacteria into the body. Recently, the development of nano-dressing containing green antibiotics has been received much attention around the world.Methods : Ingredients of essential oil of Citrus sinensis (CSEO) the used antibacterial agent were identified by GC-MS analysis. Its half-maximal inhibitory concentration (IC۵۰) against four important human bacteria strains were investigated. After that, a nanogel impregnated nanodressing was also prepared.Results : The five major components were included as limonene (۶۱.۸۳%), trans-p-۲, ۸-menthadien-۱-ol (۴.۹۵%), trans-limonene oxide (۲.۲۹ %), cis- limonene oxide (۲.۵۸ %), and trans-carveol (۲.۹۰%). The IC۵۰s were observed as follows, Staphylococcus aureus (۱.۰ mg/mL), Escherichia coli (۱۰ mg/mL), Pseudomonas aeruginosa (۴.۷ mg/mL), and Klebsiella pneumonia (۵.۸ mg/mL). The nanogel was prepared by the addition of a gelling agent (carbomer ۹۴۰ ۲%) to nanoemulsion with a particle size of ۱۲۵ ± ۴ nm. After that, the nanogel was impregnated on the surface of the electrospun nanofibers of polycaprolactone with a mean diameter of ۱۸۶ ± ۳۶ nm. Interestingly, the prepared nanodressing completely inhibited (~ ۱۰۰%) the growth of all examined bacteria at the concentration of ۱۵ mg/mL.Conclusion : The prepared prototype can be used as a potent antibacterial agent. Furthermore, this work introduced an effective and new method for the preparation of green antibacterial agents as well as antibiotic-free wound dressings.

Keywords:

Citrus sinensis , Essential oil , PCL nanofibers , Electrospinning , Nanogel , Antibacterial activity