Study on differentiation of pathogen-nonpathogen Mycobacterial infections using ESAT۶-CFP۱۰ in ELISA system
Publish place: 21th International Congress of Microbiology of Iran
Publish Year: 1399
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM21_060
تاریخ نمایه سازی: 23 مرداد 1400
Abstract:
Background and Aim : Pathogenic mycobacteria, including the causative agents of tuberculosis, are responsible for considerable morbidity and mortality worldwide. The availability of a laboratory method that can distinguish between two groups of pathogens; Mycobacterium tuberculosis complex (MTC or MTBC), nontuberculous mycobacteria (NTM) and those who have only had a history of dealing with nonpathogenic or recently vaccinated individuals is of great importance.Methods : In this regard, different strains of mycobacteria were cultured in the Dorset-Henley liquid medium and the inactivation of the cultures is carried out by the heat. The bacteria were separated from the liquid medium containing secreted proteins with EKS filters. Low molecular weight proteins in Mycobacterium tuberculosis precipitated with ammonium sulfate were purified by Sephadex-G۵۰ gel chromatography and crude antigens in other mycobacteria precipitated with TCA. Protein concentrations determined with lowry protein assay, antigens coated on the ELISA plate and the results were analyzed with SPSS software and investigated.Results : In this study, all antigens had more than ۹۲% detection ability in healthy livestock in the ELISA method. The highest specificity was related to ESAT-۶/CFP۱۰ and M. avium subsp. Paratuberculosis antigens were ۸۳.۶۶% and ۹۵.۸۳% respectively and the highest efficiency of diagnostic tests were over ۸۳% concerning these two antigens.Conclusion : It concluded that the two antigens ESAT-۶/CFP۱۰ and M. avium subsp. Paratuberculosis are suitable candidates for the design of the diagnostic ELISA system due to their sensitivity, specificity and efficiency and also reliable detection of healthy livestock from sensitized livestock.
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Authors
Anahita Bahmanjeh
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
Saeed Ataei Kachooei
Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
Mohammad Faezi Ghasemi
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
Nader Mosavari
Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
Seyed Mehdi Hassanzadeh
Vaccine Production Unit, Research & Production Complex, Pasteur Institute of Iran, Karaj, Iran