Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-۲۰۸a and MicroRNA-۱ in Cardiovascular Patients

Background: MicroRNA expression signature and reactive oxygen species (ROS) production have been associated with the development of cardiovascular diseases (CVDs). This study aimed to evaluate oxidative stress, inflammation, apoptosis, and the expression of miRNA-۲۰۸a and miRNA-۱ in cardiovascular patients. Methods: The study population included four types of patients (acute coronary syndromes (ACS), myocardial infarction (MI), arrhythmia, and heart failure (HF)), with ۱۰ people in each group, as well as a control group. Quantitative real-time PCR was performed to measure mir-۲۰۸ and miR-۱ expression, the mRNAs of inflammatory mediators (TNFα, iNOS/eNOS), and apoptotic factors (Bax and Bcl۲). XOX, MDA, and antioxidant enzymes (CAT, SOD, and GPx) were measured by ZellBio GmbH kits by an ELISA Reader. Results: The results showed significant decreases in the activity of antioxidant enzymes (CAT, SOD, and Gpx) and a significant increase in the activity of the MDA and XOX in cardiovascular patients. Significant increases in IL-۱۰, iNos, iNOS / eNOS, and TNF-α in cardiovascular patients were also observed. Also, a significant increase in the expression of miR-۲۰۸ (HF> arrhythmia> ACS> MI) and a significant decrease in the expression of miR-۱ (ACS> arrhythmia> HF> MI) were found in all four groups in cardiovascular patients. Conclusions: The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-۲۰۸a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-۱, miR-۲۰۸, and oxidative stress indices have with inflammation and apoptosis.

Keywords:

Apoptosis , Cardiovascular diseases , Inflammation , microRNA-۲۰۸a , microRNA-۱ , Oxidative stress.