Lack of an Association between a Functional Polymorphism in the MDM۲ Promoter and Breast Cancer in Women in Northeast Iran

Background: Breast cancer is one of the most common cancers among women worldwide. Tumor protein ۵۳ (TP۵۳) and its regulator the mouse double murine ۲ (MDM۲) have important roles in tumorigenesis by playing key roles in cell division and response to DNA damage. MDM۲ SNP۳۰۹ T>G (rs۲۲۷۹۷۴۴) polymorphism in the promoter region of MDM۲ gene can cause dysfunction and inactivation of TP۵۳ which promote tumor progression as a result. The aim of this study was to investigate the association between this polymorphism and risk of breast cancer in northeastern Iranian population. Material: A case-control study with ۱۲۸ breast cancer patients and ۱۴۳ healthy women was conducted. PCR-ARMS was performed to assess the MDM۲ SNP۳۰۹ T>G (rs۲۲۷۹۷۴۴) polymorphism. Results: The GG Genotype frequency between patients and controls showed no significant association between this polymorphism with breast cancer risk (p=۰.۱۱۶, OR [۹۵% CI]: ۱.۲۶۷ [۰.۶۱۶, ۲.۶۰۳]). Also, G allele frequency was not associated with breast cancer risk in studied population (p=۰.۱۴۳, OR [۹۵% CI]: ۱.۳۲۶ [۰.۹۰۸, ۱.۹۳۵]). For this polymorphism, a significant difference of ۸.۰ years in the average age of cancer diagnosis was observed between TT and TG carriers (۴۰.۵۷ versus ۴۸.۱۵ years, p = ۰.۰۲۹). Conclusion: The results of this study suggest that the SNP۳۰۹ T>G polymorphism in the MDM۲ gene may not be associated with the risk of breast cancer in an Iranian population

Keywords:

Breast cancer , Case-control study , Mouse double murine ۲ (MDM۲) , Polymorphism