Identification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m ۳, a Plant Pathogenesis-Related Protein

Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_RBMB-2-2_004

تاریخ نمایه سازی: 10 شهریور 1400

Abstract:

Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m ۱ and Cuc m ۲, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m ۳. Methods:The Cuc m ۳ cDNA has been characterized by rapid amplification of cDNA ends (RACE), which revealed a ۴۵۶ base-pair (bp) fragment encoding a ۱۵۱-amino acid polypeptide with a predicted molecular mass of ۱۶.۹۷ kDa, and identified ۷۹ and ۱۷۸ bp untranslated sequences at the ۵′ and ۳´ ends, respectively. Results: In silico analysis showed strong similarities between Cuc m ۳ and other plant pathogen-related protein ۱s from cucumber, grape, bell pepper, and tomato. Conclusion: Here we report the identification and characterization of the Cuc m ۳ cDNA, which will be utilized for further analyses of structural and allergenic features of this allergen.

Keywords:

Allergen , Cuc m ۳ , Melon , Plant pathogenesis-related protein ۱

Authors

Mojtaba Sankian

Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.

Jafar Hajavi

Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.

Malihe Moghadam

Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.

Abdol-Reza Varasteh

Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.