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Paper
Title

Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice

مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 1، شماره: 1
Year: 1391
COI: JR_RBMB-1-1_004
Language: EnglishView: 124
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Authors

Fatemeh Vahedi - Razi Vaccine & Serum Research Institute, Mashhad, Iran
Najmeh Nazari - Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Shirin Arbabi - Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Yasser Peymanfar - Razi Vaccine & Serum Research Institute, Mashhad, Iran

Abstract:

Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host genome, and elicitation of adverse immune responses. Methods: In this study, we examined the potential integration and bio-distribution of pcDNA۳.۱+PA, a new vaccine candidate with GenBank accession # EF۵۵۰۲۰۸, encoding the PA۶۳ gene, in reproductive organs of mice; ovaries and uterus in female, and testis in male. Animals of both sexes were injected intramuscularly with pcDNA۳.۱+PA. Host genome integration and tissue distribution were examined using PCR and RT-PCR two times monthly for six months. Results: RT-PCR confirmed that pcDNA۳.۱+PA was not integrated into the host genome and did not enter reproductive organs. Conclusions: This finding has important implications for the use of pcDNA۳.۱+PA plasmid as a vaccine and opens new perspectives in the DNA vaccine area.

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This Paper COI Code is JR_RBMB-1-1_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1263193/

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Vahedi, Fatemeh and Nazari, Najmeh and Arbabi, Shirin and Peymanfar, Yasser,1391,Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice,https://civilica.com/doc/1263193

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