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Title

Cloning and Expression of Cyclophilin from Platanus orientalis Pollens in Escherichia coli

Year: 1391
COI: JR_RBMB-1-1_005
Language: EnglishView: 49
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Authors

Mojtaba Sankian - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Fatemeh Vahedi - Razi Vaccine & Serum Research Institute, Mashhad, Iran
Nazanin Pazouki - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Malihe Moghadam - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Farahzad Jabbari Azad - Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abdol-Reza Varasteh - Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract:

Background: Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli for further studies and therapeutic and diagnostic purposes for allergy to P. orientalis. Methods: RNA was extracted from P. orientalis. A full-length fragment encoding cyclophilin was prepared by polymerase chain reaction amplification of the first-strand cDNA synthesized from P. orientalis RNA. The cDNA was inserted into the pET۳۲b (+) vector, and the construct transformed into E. coli Top۱۰ and BL۲۱ cells. The expressed protein was purified by the CuSO۴ method. Results: The cDNA for the cyclophilin of P. orientalis pollen was cloned, and a specific reactivity of recombinant cyclophin was confirmed by immunoblotting using sera from patients allergic to P. orientalis pollen. Conclusion: The recombinant cyclophilin has a potential for immunologic assays for evaluation of allergy to P. orientalis pollen.

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Sankian, Mojtaba and Vahedi, Fatemeh and Pazouki, Nazanin and Moghadam, Malihe and Jabbari Azad, Farahzad and Varasteh, Abdol-Reza,1391,Cloning and Expression of Cyclophilin from Platanus orientalis Pollens in Escherichia coli,https://civilica.com/doc/1263194

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Type of center: علوم پزشکی
Paper count: 8,097
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