Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats

Objective(s): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats.Materials and Methods: Fifty-six  female albino Wistar rats were divided into ۷ equal groups. Group ۱; Sham Operation, Group ۲; Ischemia, Group ۳; Ischemia + Reperfusion, Group ۴; Ischemia + Pomegranate ۰,۳۲ ml / kg (IP), Group ۵; Ischemia + Pomegranate ۰.۶۴ ml / kg, Group ۶; Ischemia + Pomegranate ۰,۳۲ ml / kg + reperfusion, Group ۷; Ischemia + Pomegranate ۰,۶۴ ml / kg + reperfusion. Three hours after ischemia and ۳ hours after reperfusion, the study was terminated. Results: While NADPH oxidase activity, MDA and TNF-α levels were significantly increased, SOD activity and GSH levels were reduced in ischemia and I/R groups. Low dose pomegranate seed oil  application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic and ischemic/reperfusion groups. At the same time, low-dose pomegranate seed oil extract reduced TNF-α levels and significantly increased antioxidant activity.Conclusion: PSO demonstrated an important therapeutic effect in the treatment of ovarian ischemia and reperfusion injury.

Keywords:

Ischemia/Reperfusion , Oxidative stress , Ovary , Pomegranate seed oil , Punicaceae , Rats