Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran
Publish place: Twenty-second Iranian Congress of Microbiology (Virtual)
Publish Year: 1400
نوع سند: مقاله کنفرانسی
زبان: English
View: 276
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM22_005
تاریخ نمایه سازی: 8 مهر 1400
Abstract:
Background and Aim : Shigellosis is a major healthcare concern in the world, especially in developing countries with poor hygiene particularly among children under ۵ years old. To investigate the antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran.Methods : In this study, ۱۵۳۰ stool samples were collected from children under ۱۵ years with diarrhea referred to teaching hospitals in Ahvaz and Abadan, southwest Iran. Shigella spp. were identified by standard biochemical tests and PCR. The antibiotic resistance pattern of all Shigella isolates was determined by the disk diffusion method and minimum inhibitory concentration (MIC) by E-test.Results : Of ۱۵۳۰ stool samples, ۹۱ (۵.۹%, ۹۱/۱۵۳۰) were positive for Shigella spp. the most common Shigella isolates were Shigella flexneri ۴۷ (۵۱.۶%, ۴۷/۱۵۳۰). Antibiotic susceptibility tests showed that the highest antibiotic resistance was related to trimethoprim-sulfamethoxazole (۸۷.۹%, ۸۰/۹۱) and ampicillin (۸۶.۸%, ۷۹/۹۱). Multiplex PCR results revealed that ۵۶% and ۸۶.۹% of Shigella isolates carried integron class I and integron class II genes, respectively. None of the isolates included the integron class III gene.Conclusion : The high prevalence of multi-drug resistance in Shigella isolates in our area increases the concerns about the dissemination of the antibiotic-resistant isolates in this bacterium.
Keywords:
Authors
Nabi Jomezadeh
Department of Microbiology, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran
Shokrollah Salmanzadeh
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences. Ahvaz. Iran
Maryam Afzali
Department of Microbiology, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran
Khadijeh Ahmadi
Department of Microbiology, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran