Detection of bla TEM and bla CTX-M in Klebsiella oxytoca isolated from patients in Khorramabad, Iran

Background and Aim : Klebsiella oxytocan is a gram-negative bacillus and an opportunistic pathogen belonging to the Enterobacteriaceae family. The aim of current study was to identify the prevalence of bla TEM and bla CTX-M genes in Klebsiella oxytoca isolated from clinical specimens (Burns, wounds, urinary tract infections, and respiratory tract infections). Methods : Klebsiella oxytoca strains screened from Klebsiella spp collected from hospitals of Khorramabad, Iran during the period from January ۲۰۱۹ to January ۲۰۲۰. After DNA extraction from all Klebsiella oxytoca clinical strains bla TEM and bla CTX-M genes detected by PCR technique. Results : A total of ۱۴۲ Klebsiella spp isolated ۳۲ Klebsiella oxytoca were collected. The results showed that ۱۹(۵۹.۳%) and ۱۶(۵۰ %) of isolates were carried bla TEM and bla CTX-M genes. Conclusion : Due to the high prevalence of bla TEM and bla CTX-M genes, their tracing in clinical specimens for infection control measures by PCR-based molecular typing method is essential for rapid and reliable identification.

Keywords:

Klebsiella spp , Klebsiella oxytoca , bla CTX-M gene , bla TEM , Enterobacteriaceae family