InvestigatedInhibitory Effect of Aqueous Extract of Pistacia atlantica on Isolates of Pathogenic Escherichia coli

Background and Aim : Today bacterial infections and antibiotic resistance are major challenge for medicines. In this regards, herbal antimicrobial properties due to their availability and low side effects, have been interested for researcher. In this study attempt to investigate the antibactrial effect of aqueous extract of Pistacia atlantica on isolates of Escherichia coli from urine specimens collected from women who suspected to urinary infection tract (UTI) in vitro.Methods : The antibacterial effect of the extract was determined by broth microdilution method on ۴۰ clinical isolates of E. coli and standard strain (ATCC ۲۵۹۲۲). The difference concentrations of P. atlantica were prepared ۰.۲-۲۰۰ mg/ml in۹۶-well plate wells, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined by using colony count on blood agar medium. The collected data were statistically analyzed by SPSS (V. ۲۲) software using Chi-square and t-test.Results : The Mean± SD of MIC and MBC of P. atlantica aqueous extract were determinate ۴۶.۸±۳.۸ and ۹۳.۷±۷۷.۳ on clinical isolates respectively. The results showed that highest antibacterial effects in ۵۰ mg/ µl concentration on clinical isolates of E. coli. Also, the result did not show significantly difference between MIC and MBC of ATCC (MIC and MBC were ۱۰۰ and ۲۰۰ mg/µl respectively) strain with clinical isolates (p= ۱.۰).Conclusion : Based on the results, the aqueous extract of P. atlantica exhibited antibacterial potency against on the clinical isolates of E. coli and could be affective as natural antimicrobial agent.

Keywords:

Herbal , Pistacia atlantica , Antibacterial , E. coli