Comparison of Cattle Serum Antibody Responses to Five Different Mycobacterial Antigens in An ELISA System

Background and Aim : In view of the fact that the presence of common diseases between humans and livestock caused by Mycobacterium tuberculosis complex (MTBC), and nontuberculous mycobacteria (NTM), Such as Johne's disease in animals and Crohn's disease in humans, early detection of mycobacteria can help Control of health problems and huge economic losses in Iranian livestock. New methods of diagnoIn view of the fact that the presence of common diseases between humans and livestock caused by Mycobacterium tuberculosis complex (MTBC), and nontuberculous mycobacteria (NTM), Such as Johne's disease in animals and Crohn's disease in humans, early detection of mycobacteria can help Control of health problems and huge economic losses in Iranian livestock. New methods of diagnosis are an ELISA method that is easy, fast, and useful. In this research, we attempted to evaluate different crude antigens obtained from mycobacterial species using an indirect ELISA test. The purpose of this assessment is to detect the infection of different mycobacteria in infected livestock.sis are an ELISA method that is easy, fast, and useful. In this research, we attempted to evaluate different crude antigens obtained from mycobacterial species using an indirect ELISA test. The purpose of this assessment is to detect the infection of different mycobacteria in infected livestock.Methods : Five different strains of Mycobacteria (M. tuberculosis, M. phlei, M. bovis, M. avium subsp. Paratuberculosis, and M. bovis AN۵) were cultured and precipitated by TCA ۴%. The SDS-PAGE of crude antigens of different mycobacteria was accomplished. Protein concentrations determined with Lowry protein assay. After preparing the crude antigens of mycobacteria, the ELISA test was performed, the results of the ELISA test were compared with the PPD skin test and data analysed with SPSS software.Results : Study showed, all five strains had more than ۹۲% detection ability in healthy animals. The highest sensitivity of ELISA tests to M. bovis AN۵ antigen is greater than ۸۳% and the highest diagnostic specificity of M. avium subsp. Paratuberculosis is ۹۵.۸۳% and the highest efficiency of diagnostic tests is over ۸۳% concerning this antigen.Conclusion : In conclusion, this study concludes that M. avium subsp. Paratuberculosis and M. bovis AN۵ antigens are suitable candidates for the design of diagnostic ELISA due to their sensitivity, specificity, and efficiency.

Keywords:

Mycobacteria , NTM , PPD , ELISA