The effects of bortezomib on the ovariectomy applied rat uterus: A histopathological, stereological and immunohistochemical study

Objective(s): In this study, potential protective effects of Bortezomib (Bort), as a proteasome inhibitor, were investigated on the uterus of ovariectomized rats by histological, morphometric and immunohistochemical methods.Materials and Methods: In this study, ۱۸ Sprague dawley strain female rats (۱۲ weeks old, ۲۵۰-۳۰۰ g body weight) were used. Animals in the control group (Cont, n=۶) were not exposed to any treatment. Ovariectomy was performed on the experimental groups. They (n=۱۲) were divided into ovariectomy (Ovt, n=۶) and Bortezomib (Bort, n=۶) subgroups. Twelve weeks later, the rats were perfused. Then, uterine tissues were removed and examined by morphometrical, and light and electron microscopy methods. In addition, immunoreactivity of nuclear factor-kappa (NF-κB) was evaluated.Results: Morphometric and histopathological evaluations showed that Bort was effective in the uterus and protects the layer structures and the cells.Conclusion: In the light of these findings, we suggest that for proteasome inhibitor particularly Bort is thought to be useful through proteasome inhibition and NF-κB pathway.

Keywords:

Bortezomib Microscopy Ovariectomy Rat Stereology , Uterus