An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue

Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-۲۹, HCT-۱۱۶, RKO and healthy cell line MRC۵ were cultured by different seeding procedure. After cell viability assay, changes in caspase ۳ enzyme activity and the level of phosphatidylserine were measured.Results: For cell viability assay, a ۴۸ hr incubation period was chosen to seed all cells together. There was a ۱.۳۶-fold decrease in caspase ۳ enzyme activity by co-treatment of RKO and MSCs in addition to ۲.۰۲-fold decrease in HT-۲۹ and MSCs co-treatment, and ۱.۱۰۳-fold increase in HCT-۱۱۶ and MSCs. The results demonstrated that HCT-۱۱۶ led to the highest rate of apoptotic cell death (۷.۵%) compared with other cells.Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.

Keywords:

Apoptosis , Colon cancer , Stem cells , Cell death