Evaluation of Tnf-α and Il-۶ mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol

Objective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-۶ (IL-۶) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated. Materials and Methods: Fifteen female Wistar rats (۲۱ days old) were divided into three groups (n=۵): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate ۱ mg/۱۰۰ g body weight subcutaneously for ۳۵ days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol ۱۰ mg/kg intraperitoneally for ۲۸ days. The expression of Tnf-α and Il-۶ mRNAs in adipose tissues was determined by the qRT-PCR method. Results: The Il-۶ mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (P<۰.۰۵). Tnf-α and Il-۶ mRNA expression in visceral and subcutaneous adipose tissues of polycystic ovarian rats was similar to controls. The expression of Tnf-α mRNA in subcutaneous adipose tissue and Tnf-α and Il-۶ mRNAs in the visceral adipose tissue of the PCO-model-resveratrol group were lower than PCOS rats (P<۰.۰۵). Conclusion: Increased expression of Il-۶ mRNA in the visceral adipose tissue of polycystic ovarian rats may be one cause of insulin resistance observed in them and resveratrol as an anti-inflammatory and anti-hyperglycemic agent may decrease the risk of diabetes by reduction of expression of pro-inflammatory cytokines TNF-α and IL-۶ in PCOS patients.

Keywords:

Adipose tissue , IL-۶ , Polycystic ovary – , syndrome , Rat , Resveratrol , TNF-α