Evaluation of Tnf-α and Il-۶ mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-21-2_008

تاریخ نمایه سازی: 27 مهر 1400

Abstract:

Objective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-۶ (IL-۶) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated. Materials and Methods: Fifteen female Wistar rats (۲۱ days old) were divided into three groups (n=۵): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate ۱ mg/۱۰۰ g body weight subcutaneously for ۳۵ days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol ۱۰ mg/kg intraperitoneally for ۲۸ days. The expression of Tnf-α and Il-۶ mRNAs in adipose tissues was determined by the qRT-PCR method. Results: The Il-۶ mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (P<۰.۰۵). Tnf-α and Il-۶ mRNA expression in visceral and subcutaneous adipose tissues of polycystic ovarian rats was similar to controls. The expression of Tnf-α mRNA in subcutaneous adipose tissue and Tnf-α and Il-۶ mRNAs in the visceral adipose tissue of the PCO-model-resveratrol group were lower than PCOS rats (P<۰.۰۵). Conclusion: Increased expression of Il-۶ mRNA in the visceral adipose tissue of polycystic ovarian rats may be one cause of insulin resistance observed in them and resveratrol as an anti-inflammatory and anti-hyperglycemic agent may decrease the risk of diabetes by reduction of expression of pro-inflammatory cytokines TNF-α and IL-۶ in PCOS patients.

Authors

Mahnaz Ghowsi

Department of Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran

Homayoun Khazali

Department of Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran

Sajjad Sisakhtnezhad

Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran

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