Expression of pro-inflammatory genes in lesions, spleens and blood neutrophils after burn injuries in mice treated with silver sulfodiazine
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 20، Issue: 7
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-20-7_006
تاریخ نمایه سازی: 28 مهر 1400
Abstract:
Objective(s): It is now supposed that cytokines released during the burn injuries have a great impact on the immunological and pathological responses after the burn. The main objective of this study was to investigate the expression of some pro-inflammatory genes in the wound, spleen and blood neutrophils during the healing process of burn wounds in a murine model. Materials and Methods: The expression of ten pro-inflammatory genes were examined in wounds, spleens and blood neutrophils of mice with burn injuries treated with either silver sulfodiazine or phosphate-buffered saline (PBS) using RT-PCR at the end of the first and second weeks after injuries. Results: None of the pro-inflammatory genes were expressed in the skin, spleen and blood neutrophils of healthy mice. In the group control, IL-۱۲P۳۵, IL-۱۲P۴۰, CCR۵, IL-۱β and IFN- γ were expressed in the spleen and blood neutrophils in the first week. Instead, CCL۵, CCR۵, IL-۱β and IFN- γ were expressed in the wound, but in the second week, the expression of the genes became similar. In the test group, in the first week, TNF-α, IL-۱۲P۳۵, IL-۱۲P۴۰ and IL-۱β were expressed in the lesions, CCL۴, IL-۱α, IL-۱۲P۳۵, IL-۱۲P۴۰, CCR۵ and IFN- γ were expressed in the spleen and no pro-inflammatory gene expression was detected in blood neutrophils. Conclusion: IL-۱β and IFN- γ are expressed in wound, spleen and neutrophils of untreated mice, but not in silver sulfodiazine treated mice. Hence, treatment with silver sulfodiazine suppressed the expression of pro-inflammatory genes in some stages of healing.
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Authors
Soheyla Akhzari
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
Hossein Rezvan
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
Seyed Masoud Zolhavarieh
Department of Clinical Sciences, Faculty of Veterinary Science, Hamedan, Iran
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