Cytotoxic and apoptotic effects of different extracts of Artemisia biennis Willd. on K۵۶۲ and HL-۶۰ cell lines

Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-20-2_009

تاریخ نمایه سازی: 28 مهر 1400

Abstract:

Objective(s): Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies. Materials and Methods:In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol:water (۱:۱ v/v) extracts of A. biennis Willd. on two cancer human cell lines (K۵۶۲ and HL-۶۰) and J۷۷۴ as normal cells. Results: CH۲Cl۲ extract was found to have the highest anti-proliferative effect on cancer cells. IC۵۰ values obtained in AlamarBlue® assay for CH۲Cl۲ extract were ۶۴.۸۶ and ۵۴.۳۱ µg/ml on K۵۶۲ and HL-۶۰ cells respectively. In flow cytometry histogram of the cells treated with CH۲Cl۲ extract, sub-G۱ peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CH۲Cl۲ extract after ۴۸ hr contact with cells. Conclusion: The results can corroborate the cytotoxic and apoptotic effects of the CH۲Cl۲ extract of A. biennis on the K۵۶۲ and HL-۶۰ cancer cell lines.

Authors

Zahra Tayarani-Najaran

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Farideh-Sadat Makki

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Nafiseh-Sadat Alamolhodaei

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Mahdi Mojarrab

Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

Seyed Ahmad Emami

Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Bora KS, Sharma A. The genus Artemisia: a comprehensive review. ...
  • Gilani AH, Yaeesh S, Jamal Q, Ghayur MN. Hepatoprotective activity ...
  • Aniya Y, Shimabukuro M, Shimoji M, et al. Antioxidant and ...
  • Kordali S, Cakir A, Mavi A, Kilic H, Yildirim A. ...
  • Kordali S, Kotan R, Mavi A, Cakir A, Ala A, ...
  • Abad MJ, Bedoya LM, Apaza L, Bermejo P. The Artemisia ...
  • Mozaffarian V. Flora of Iran, No ۵۹: Compositae (Anthemideae & ...
  • Emami SA, Aghazari F. Les Phanerogames Endemiques de la Flore ...
  • Tan RX, Tang HQ, Hu J, Shuai B. Lignans and ...
  • Choi E, Park H, Lee J, Kim G. Anti-cancer, antiobesity, ...
  • Taghizadeh Rabe SZ, Mahmoudi M, Ahi A, Emami SA. Antiproliferative ...
  • Tin AS, Sundar SN, Tran KQ, Park AH, Poindexter KM, ...
  • Lee JG, Kim JH, Ahn JH, Lee KT, Baek NI, ...
  • Park EY, Lee KW, Lee HW, Cho YW, Baek NI, ...
  • Ju HK, Lee HW, Chung KS, Standardized flavonoid-rich extract of ...
  • Mozaffarian V. A Dictionary of Iranian Plant Names, p.۵۶ Tehran: ...
  • Nematollahi F, Rustaiyan A, Larijani K, Nadimi M. Essential oil ...
  • Emami A, Zamani Taghizadeh Rabe SH, Ahi A, Mahmoudi M. ...
  • Emami A, Zamani Taghizadeh Rabe SH, Ahi A, Mahmoudi M. ...
  • Hatami T, Emami SA, Miraghaee SS, Mojarrab M. Total phenolic ...
  • Mousavi SH, Tayarani-Najaran Z, and Hersey P. Apoptosis, from signaling ...
  • Nobili S, Lippi D, Witort E, Donnini M, Bausi L, ...
  • Russo GL. Ins and outs of dietary phytochemicals in cancer ...
  • Kim MJ, Kim DH, Na HK, Oh TY, Shin CY, ...
  • Lv W, Sheng X, Chen T, Xu Q, Xie X. ...
  • Martins LM, Mesner PW, Kottke TJ, Basi GS, Sinha S, ...
  • O’Brien J, Wilson I, Orton T, and Pognan F. Investigation ...
  • Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. ...
  • Shu L, Cheung KL, Khor TO, Chen C, Kong AN. ...
  • Wang C, and Youle RJ. The role of mitochondria in ...
  • Rao RV, Ellerby HM, Bredesen DE. Coupling endoplasmic reticulum stress ...
  • Letai A. Pharmacological manipulation of Bcl-۲ family members to control ...
  • Rashid S, Rather MA, Shah WA, Bhat BA. Chemical composition, ...
  • Spies L, Koekemoer TC, Sowemimo AA, Goosen ED, Van De ...
  • Piao GC, Li YX, Yuan HD, Jin GZ. Cytotoxic extract ...
  • Wegiera M, Smolarz HD, Jedruch M, Korczak M, Koproń K. ...
  • Shafi G, Hasan TN, Syed NA, Al-Hazzani AA, Alshatwi AA, ...
  • Baykan Erel S, Gökhan Şenol S, Aydin Köse F, Ballar ...
  • Judzentiene A, Budiene J, Gircyte R, Masotti, V, Laffont-Schwob I. ...
  • Lee TK, Vairappan CS. Antioxidant, antibacterial and cytotoxic activities of ...
  • Efferth T, Herrmann F, Tahrani A, Wink M. Cytotoxic activity ...
  • Tilaoui M, Mouse HA, Jaafari A, Aboufatima R, Chait A, ...
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