G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease

Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of BMSCs through the tail vein. Materials and Methods: We choose ۲۵ male Wistar rats (۲۰۰–۲۵۰ g) were lesioned by ۶-OHDA injected into the left substantia nigra, pars compacta (SNpc). G-CSF (۷۰ µg/kg/day) was given from the ۷th day after lesion for five days. The BMSCs (۲×۱۰۵) were injected through the dorsal tail vein on the ۷th day after lesion. Results:The number of rotations was significantly lower in the stem cell therapy group than in the control group. In the third test in the received G-CSF and G-CSF+stem cells groups, animals displayed significant behavioral recovery compared with the control group (P<۰.۰۵). There was a significant difference in the average of dopaminergic neurons in SNpc between the control group and G-CSF and G-CS+stem cells groups. We didn't detect any labeling stem cells in SNpc. Conclusion: G-CSF can't mobilize low amounts of exogenous BMSCs from the blood stream to injured SNpc. But G-CSF (۷۰ µg/kg) is more neuroprotective than BMSCs (۲×۱۰۵ number of BMSCs). Results of our study suggest that G-CSF alone is more neuroprotective than BMSCs.

Keywords:

Bone marrow stem cells , Dopaminergic neurons , Granulocyte colony stimulating factor , Parkinson