Anti-hyperglycaemic and antioxidant effects of Bidens tripartita and quantitative analysis on its active principles
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-19-10_011
تاریخ نمایه سازی: 30 مهر 1400
Abstract:
Objective(s): Bidens species are used for their antidiabetic properties traditionally in many countries. Aim of this study is to evaluate hypoglycaemic and antidiabetic activity of Bidens tripartita extract and to identify its active compounds through bioactivity guided isolation technique. Materials and Methods: Hypoglycaemic effects of B. tripartita extract and its sub-extracts were investigated in normal and glucose-hyperglycaemic rats. Streptozotocin induced diabetic rats were used to examine antidiabetic activity of the extract and its sub-extracts after acute and sub-acute administration. Additionally, in vitro enzyme inhibitory and antioxidant activities were evaluated. HPLC analyses were carried out to determine the active constituents of the extract and its sub-extracts. Results: Through in vivo bioactivity-guided fractionation process, ethyl acetate and n-buthanol sub-extracts were found to have potent antidiabetic activity. In vitro enzyme inhibitory activities of the same sub-extracts were found to be potent. The highest total phenol, flavonoid contents and radical scavenging activity was determined in ethyl acetate sub-extract. According to LC-MS analyses, chlorogenic acid, luteolin and ۷-O-glucoside of luteolin (cynaroside) were determined as the main components of the active sub-extracts. Conclusion: According to our results, B. tripartita has potent antidiabetic activity and its active constituents might be beneficial for diabetes and its complications.
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Authors
Nilüfer Orhan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, ۰۶۳۳۰, Etiler, Ankara, Turkey
Ülkü Gökçen İçöz
Republic of Turkey Social Security Institution, ۰۶۵۲۰, Balgat, Ankara, Turkey
Levent Altun
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, ۰۶۵۰۰, Tandogan, Ankara, Turkey
Mustafa Aslan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, ۰۶۳۳۰, Etiler, Ankara, Turkey
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