The Expression implication of GDNF in ventral horn and associated remote cortex in rhesus monkeys with hemisected spinal cord injury
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 19، Issue: 9
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-19-9_008
تاریخ نمایه سازی: 30 مهر 1400
Abstract:
Objective(s): Glial cell line-derived neurotrophic factor (GDNF) can effectively promote axonal regeneration,limit axonal retraction,and produce a statistically significant improvement in motor recovery after spinal cord injury (SCI). However, the role in primate animals with SCI is not fully cognized. Materials and Methods:۱۸ healthy juvenile rhesuses were divided randomly into six groups, observed during the periods of ۲۴ hr, ۷ days, ۱۴ days, ۱ month, ۲ months, and ۳ months after T۱۱ hemisecting. The GDNF localization, changes in the injured region, and the remote associate cortex were detected by immunohistochemical staining. Results: Immunohistochemical staining showed that GDNF was located in the cytoplasm and the neurite of the neurons. Following SCI, the number of GDNF positive neurons in the ventral horn and the caudal part near the lesion area were apparently reduced at detected time points (P<۰.۰۵). Moreover, the number in the rostral part of the ventral horn in ۷ day, ۱۴ day, and ۱ month groups were fewer than those in the caudal part. Importantly, in the contralateral cortex motor area, the positive neurons decreased sharply after hemi-SCI, while gradually increased and went back to normal in ۳ months after hemi-SCI. Conclusion: To sum up, GDNF disruption in neurons occurred after SCI especially in cortex motor area. Intrinsic GDNF in the spinal cord, plays an essential role in neuroplasticity. Thereafter extrinsic GDNF supplementing may be a useful strategy to promote recovery after SCI.
Keywords:
GDNF (glial cell line-derived neurotrophic factor) , Hemi-SCI (hemisection of spinal cord injury) , Macaca mulatta , Motor cortex , Spinal cord ventral horn
Authors
De-Lu Qiu
Laboratory of Anesthesia and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ۶۱۰۰۴۱, China
Ting-Hua Wang
Laboratory of Anesthesia and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ۶۱۰۰۴۱, China
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