Immunomodulatory activities of gemifloxacin in mice

Objective(s):Gemifloxacin is a broad spectrum antibiotic and has shown excellent coverage against a wide variety of microorganisms. In this study, an attempt was made to evaluate the immunomodulatory potential of gemifloxacin in male swiss albino mice in vivo. Materials and Methods: Three doses of gemifloxacin ۲۵ mg/kg, ۵۰ mg/kg and ۷۵ mg/kg were used intraperitoneally (IP) for the evaluation of immune responses in mice. Delayed type hypersensitivity (DTH), heamagglutination assay, jerne hemolytic plaque formation assay and cyclophosphamide induced neutropenia assay were performed to evaluate the effect of gemifloxacin on immune responses. Results: DTH assay has shown the significant immune suppressant potential of gemifloxacin at ۲۵ mg/kg dose and ۷۵mg/kg dose. Total leukocyte count (TLC) has shown decrease in leukocyte count (P<۰.۰۵) in drug treatment groups before cyclophosphamide administration and significant decrease (P<۰.۰۰۱) in leukocyte count after cyclophosphamide administration as compared to negative control group. Differential leukocyte count (DLC) has shown significant decrease (P<۰.۰۰۱) in percentage count of lymphocytes in ۷۵ mg/kg treatment group in leukopenic mice while increase (P<۰.۰۱) in monocytes percentage in ۵۰ mg/kg treatment group in leukopenic mice and increase in neutrophil percentage count (P<۰.۰۵) in all treatment groups was observed after cyclophosphamide administration. Humoral immune response is shown to be suppressed in dose dependent manner by both heamagglutination titre values (P<۰.۰۰۱) and jerne hemolytic plaque formation assay (P<۰.۰۰۱). Conclusion: The results of this work clearly demonstrate that gemifloxacin has significant immunomodulatory potential.

Keywords:

Gemifloxacin , Immunomodulatory , Immune response , Cell mediated , Humoral