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Design of cocktail peptide vaccine against Cytomegalovirus infection

Publish Year: 1395
Type: Journal paper
Language: English
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JR_IJBMS-19-4_015

Index date: 22 October 2021

Design of cocktail peptide vaccine against Cytomegalovirus infection abstract

Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pichia pastoris yeast system were studied. Materials and Method: In this study, four immune dominant sequences in pp65 protein and 3 immuno dominant sequences in gB protein were selected according to literature review. Peptide linker -GGGGS- was used for construction of fusion peptide. This fusion peptide was cloned in the pPICZαA expression vector and transfected into P. pastoris host cells. Results: Dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) techniques showed that a high level of pp65-gB-Fc fusion peptide was expressed. Conclusion: This CMV pp65-gB-Fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.

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Design of cocktail peptide vaccine against Cytomegalovirus infection authors

Samira Tabaei

Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Baratali Mashkani

Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Arezoo Esmaili

Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Reza Karimi

Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Saeid Amel Jamehdar

Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Science, Mashhad, Iran

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