Design of cocktail peptide vaccine against Cytomegalovirus infection
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 19، Issue: 4
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-19-4_015
تاریخ نمایه سازی: 30 مهر 1400
Abstract:
Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp۶۵ and gB fusion peptides and Fc domain of mouse IgG۲a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pichia pastoris yeast system were studied. Materials and Method: In this study, four immune dominant sequences in pp۶۵ protein and ۳ immuno dominant sequences in gB protein were selected according to literature review. Peptide linker -GGGGS- was used for construction of fusion peptide. This fusion peptide was cloned in the pPICZαA expression vector and transfected into P. pastoris host cells. Results: Dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) techniques showed that a high level of pp۶۵-gB-Fc fusion peptide was expressed. Conclusion: This CMV pp۶۵-gB-Fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.
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Authors
Samira Tabaei
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Baratali Mashkani
Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Arezoo Esmaili
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Reza Karimi
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Saeid Amel Jamehdar
Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Science, Mashhad, Iran
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