qnr Prevalence in Extended Spectrum Beta-lactamases (ESBLs) and None- ESBLs Producing Escherichia coli Isolated from Urinary Tract Infections in Central of Iran

Objective(s) Extensive use of quinolones has been associated with raising level of resistance. In the current, we focused on assessing the prevalence of Escherichia coli resistance to quinolones and frequency of qnrA, qnrB and qnrS in non ESBLs (extended spectrum beta-lactamases) and ESBLs producing E. coli with blaSHV and blaTEM. Materials and Methods One hundred and fifty E. coli isolates were identified during Mar. ۲۰۰۷ to Apr. ۲۰۰۸ in Milad (Tehran) hospital. They were tested for ESBLs production as well as quinolone resistance. PCR was performed for detection of blaSHV and blaTEM as well as qnrA, B and S. Results Of ۱۵۰ isolates, forty-two (۲۸%) ESBLs producing and one hundred and eight (۷۲%) non-ESBLs producing E. coli were identified. ۶۴.۲% (n= ۲۴) of E. coli producing ESBLs and ۴.۶۲% (n= ۵) of non-ESBLs E. coli were resistance to ciprofloxacin. ۹۵.۲% (n= ۴۰) and ۲۶.۱% (n= ۱۱) of the isolates harbored blaTEM and blaSHV, respectively. ۲۳.۸% (n= ۱۰) had both genes. ۳۷.۵% (n= ۹) and ۲۰.۸% (n= ۴) of ESBLs producing E. coli were positive for qnrA and qnrB respectively. qnrS was not identified in any isolate. Conclusion Our study showed high frequency of ESBLs producing E. coli as well as quinolone resistance genes (qnrA, qnrB) in Milad hospital.

Keywords:

Ciprofloxacin , Iran , Resistance