Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 4
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-18-4_006
تاریخ نمایه سازی: 3 آبان 1400
Abstract:
Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD۴+ and CD۸+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp۳۱۴۸-۷۴ and BP۲۶۸۷-۱۱۱ fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL۲۱-DE۳ and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in ۷۶.۵% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies.
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Authors
Jafar Amani
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Amir Ghasemi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Reza Ranjbar
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Mahdi Shabani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mahdi Zandemami
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Reza Golmohammadi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
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