Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 18، Issue: 3
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-18-3_013
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s):The neuroprotective effect offruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC۱۲ cells as an appropriate in vitro model of diabetic neuropathy was investigated. Materials and Methods: Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-۳, a biochemical parameter of cellular apoptosis, was measured by western blot analysis. Results: Our data showed that a ۴-fold elevation in glucose levels within the medium significantly reduced cell viability, increased intracellular ROS and caspase-۳ activation in PC۱۲ cells after ۲۴ hr. Incubation of the high glucose medium cells with ۳۰۰-μg/ml Z. jujuba fruit (ZJF) extract decreased the high glucose-induced cell toxicity and prevented caspase-۳ activation and excited ROS generation. Conclusion: Thus, we concluded that the aqueous extract of Z. jujuba protects against hyperglycaemia-induced cellular toxicity. This could be associated with the prevention of ROS generation and neural apoptosis. Moreover, the results suggest that the ZJF has a therapeutic potential to attenuate diabetes complications such as neuropathy.
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Authors
Ayat Kaeidi
Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Majid Taati
Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Zahra Hajializadeh
Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
Faranak Jahandari
Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
Marzieh Rashidipour
Young Researchers Club, Khoramabad Brach, Islamic Azad University, Khoramabad, Iran
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