Ccr۲-۶۴i and Ccr۵ Δ۳۲ Polymorphisms in Patients with Late-Onset Alzheimer’s disease; A Study from Iran (Ccr۲-۶۴i And Ccr۵ Δ۳۲ Polymorphisms in Alzheimer’s disease)

Objective(s) Alzheimer’s disease (AD) is a complex disease with multifactorial etiology. Inflammation has been proven to have an important role in the pathogenesis of AD. Both CCR۲ and CCR۵ genes expression increase in AD patients comparing to control subjects. CCR۵ gene encodes a protein which is a member of the beta chemokine receptors family of integral membrane proteins. CCR۵-Δ۳۲ is a genetic variant of CCR۵ and is characterized by the presence of a ۳۲-bp deletion in the coding region of the gene, which leads to the expression of a nonfunctional receptor, and the CCR۲-۶۴I has a change of valine to isoleucine at codon ۶۴, in the first transmembrane domain. It has been proved that both genes have important roles in different stages of inflammation. Materials and Methods The frequencies of CCR۵∆۳۲ and CCR۲-۶۴I variations were determined in ۱۵۶ AD patients and ۱۶۱ control subjects using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods, and the results were compared among AD and healthy controls. Results Statistical analysis showed no significant difference in the distributions of CCR۵∆۳۲ and CCR۲-۶۴I between the AD patients and healthy controls (P> ۰.۰۵). Stratifying the samples by gender, genetic background and presence of ApoEε۴ allele showed no significant effect on the distributions of CCR۵∆۳۲ and CCR۲-۶۴I        (P> ۰.۰۵). Conclusion Our study did not show an association between CCR۵∆۳۲ and CCR۲-۶۴I variations and AD in the Iranian population. Further confirmatory studies with bigger number of samples are recommended.

Keywords:

Alzheimer’s disease , Genetic Association study , CCR۲ , CCR۵ , Inflammation , Iranian Population