Association of ADAM۳۳ gene polymorphisms with allergic asthma

Objective(s): Asthma results from the interaction between genetic and environmental factors. ADAM۳۳ gene on chromosome ۲۰p۱۳ is associated with asthma and airway hyperresponsiveness. Materials and Methods: This is a case-control study, where four SNPs S۱ (rs۳۹۱۸۳۹۶), T۱ (rs۲۲۸۰۰۹۱), T۲ (rs۲۲۸۰۰۹۰), V۴ (rs۲۷۸۷۰۹۴) of ADAM۳۳ gene have been assessed in patients with allergic asthma and normal controls (۹۵ patients and ۸۶ normal). Blood samples of these participants have been genotyped by PCR and the RFLP method. Results: There was no association between asthmatic patients and polymorphisms of alleles, genotypes and haplotypes of the ADAM۳۳ gene. When categorizing the asthmatic patients in severe, moderate and mild groups, associations in the subcategories of asthmatic patients were found. There were associations between polymorphisms of C allele of T۱ SNP with severe asthmatic patients and G allele of V۴ SNP with moderate asthmatics respectively (P=۰.۰۰۶, P=۰.۰۱). There was a significant association between sensitivity to mite and polymorphism of C allele of T۱ SNP (P=۰.۰۲). Besides, there was a significant association between sensitivity to weeds and genotype GG of V۴ SNP (P=۰.۰۵). Conclusion: Polymorphisms of ADAM۳۳ gene might be associated with severe asthma and sensitivity to aeroallergens in northeast of Iran, but further studies are needed to determine the polymorphisms in this area and other regions of our country.

Keywords:

ADAM۳۳ , Allergic asthma , Genetic , SNP