Comparison of the Ex Vivo Expansion of UCB-Derived CD۳۴+ in ۳D DBM/MBA Scaffolds with USSC as a Feeder Layer
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-16-10_007
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s): Ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (UCB)-CD۳۴+ cells for bone marrow transplantation. For this purpose demineralized bone matrix (DBM) and mineralized bone allograft (MBA) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal progenitor cells (MPCs) - unrestricted somatic stem cells (USSCs) - as a feeder layer. Materials and Methods: USSCs were isolated and characterized by morphological and immunological analysis then seeded on both scaffolds as a feeder layer. UCB-CD۳۴+ were isolated by MACS method and were co-culture expanded by USSC in ۳D and ۲D environments. After ۳ weeks expansion, cells were counted and were assessed by karyotype, flow cytometry, clonogenic activity, and long-term culture-initiating cells (LTC-IC). Results: Co-culture expansion in DBM and MBA was ۲۹.۲۲-fold and ۲۷.۷۷-fold, no significant differences in colony and LTC-IC were obtained. Maximum number of colonies belonged to the day ۱۴ with the ۷۳% CFU-GM (Colony Forming Unit- Granulocyte/Macrophage) in contrast to the day ۰ which was BFU-E/CFU-E (Burst/Colony Forming Unit-Erythroid). Flow cytometry indicated that the percentage of CD۳۴+ marker was decreased in USSC co-culture and the highest percentage was observed in simple ۲D culture. Conclusion: Because of acid extraction in the DBM production process, mineral materials were removed and the protein background that was more flexible was presented. Therefore these results suggest that USSC-DBM can be a suitable ex vivo mimicry niche by intensifying of surface/volume ratio and supporting the stem cell differentiation and expansion.
Keywords:
DBM Ex vivo expansion MBA scaffold UCB-CD۳۴+ USSC cells
Authors
Zahra Sadat Hashemi
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mahdi Forouzandeh Moghadam
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Masoud Soleimani
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
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