Automated ribotyping and antibiotic resistance determining of Bacillus spp from conjunctiva of diabetic patients
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 17، Issue: 2
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-17-2_011
تاریخ نمایه سازی: 5 آبان 1400
Abstract:
Objective(s): We aimed to characterize the phenotype and genotype of Bacillus spp isolated from diabetic patients’ eyes, by studying the drug sensitivity patterns with a disc-diffusion method. Materials and Methods: Fifty eyes of ۲۵ patients with type II diabetes mellitus, with at least ۱۰ years of diabetes history, were included in the study. We analyzed the eyes for the presence of Bacillus spp.; presumptive isolates were identified by morphological, and biochemical tests, and confirmed by the VITEK system. Automated EcoRIribotyping was performed with a RiboPrinter® Microbial CharacterizationSystem. We determinedthe antibiotic resistance of the isolates by the Kirby–Bauer disc diffusion test. Results: Seven out of ۲۵ patients were on insulin treatment; ۷ on oral anti-diabetic medication; and ۱۱ on combination therapy of insulin and oral medications.Among the ۲۸ Bacillus spp isolates, ۱۴ were B. cereus, ۱۱ were B. pumilus, ۲ were B. mojavensis and ۱ was B. subtilis. Almost all the strains were either resistant or multiresistant, particularly towards cefuroxime, methicillin, and ceftazidime. Conclusion: Diabetic patients seem to be more prone to B. cereus infections than healthy individuals. It would be greatly beneficial to understand and recognize the prevalence of microorganisms and their resistance patterns for better outcome in ocular surgeries.
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Authors
Sertaç Argun Kıvanç
Erzurum Region Education and Research Hospital, Department of Ophthalmology, Erzurum, Turkey
Merih Kıvanç
Anadolu University, Faculty of Science, Department of Biology, Eskisehir Turkey
Gülay Güllülü
Armedica Eye Hospital, Kocaeli, Turkey
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