Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 17، Issue: 4
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
View: 210
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
این Paper در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-17-4_006
تاریخ نمایه سازی: 5 آبان 1400
Abstract:
Objective(s):In insulin resistance, the insulin action in liver, muscles and adipocytes decreases and result in hyperglycemia, dyslipidemia and hyperinsulinemia. In this study we evaluate the effect of Zataria multiflora extract on insulin sensitivity in high fructose fed insulin resistant rats, since this extract was shown antihyperglycemic effect in streptozotocin induced diabetes in rats. Materials and Methods:Experimental rats were fed with high fructose diet for ۶ weeks and then were treated with Z. multiflora extractor a pioglitazone solution for ۲ weeks. Blood and tissue samples were collected for analysis at the end of two weeks. Blood glucose, serum level of triglyceride and cholesterol were measured by auto analyzer. Insulin and adiponectin levels were assayed by enzyme-linked immunosorbent assay (ELISA) method. Plasma free fatty acids profile was studied by gas chromatography. Peroxisome proliferator activated receptor (PPAR.γ) and Glucose transporter type ۴ (GLUT.۴) gene expressions were assessed by real time polymerase chain reaction (PCR) and western blotting. Results: Animals were treated by Z. multiflora extractshowed insulin (۴۳±۱۱pmol/l), adiponectin (۵.۳±۰.۵ μg/ml), glucose (۱۴۴±۹.۸ mg/dl), and triglyceride (۱۲۰±۱۰ mg/dl) levels significantly improved as compare with the control group [insulin (۱۳۷±۳۴ pmol/l), adiponectin (۳.۹±۰.۱۵ μg/ml), glucose (۱۸۷±۱۵mg/dl), and triglycerides (۲۱۷±۱۸ mg/dl)]. PPARγ protein level, also significantly increased in Zataria multiflora treated group. Conclusion: This study demonstrates the beneficial effects of Zataria multiflora extract on insulin resistance in rats fed with a high-fructose diet through at least three mechanisms including direct insulin like effect, increasing in adiponectin and of PPARγ protein expression.
Keywords:
Authors
Abbas Mohammadi
Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Ahmad Gholamhoseinian
Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Hossein Fallah
Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :