Net Analyte Signal Standard Addition Method for the Simultaneous Determination of Hydrochlorothiazide and Triamterene in Pharmaceutical Formulations

A net analyte signal standard addition method (NASSAM) is presented for the simultaneous determination of Hydrochlorothiazide and Triamterene by spectrophotometry. The method combines the advantages of the standard addition method with the net analyte signal concept Which enables the extraction of information concerning a certain analyte from spectra of multi-component mixtures. This method has some advantages such as: the use of a full spectrum realization in a signal step, therefore, it does not require calibration and prediction steps and only a few measurements are required for the determination.The simultaneous determination of Hydrochlorothiazide and Triamterene was performed in Britton-Robinson buffer (pH 7). Three pharmaceutical compounds were determined in concentration range of 11 -71 μmol L-1.

Net Analyte Signal Standard Addition Method for the Simultaneous Determination of Hydrochlorothiazide and Triamterene in Pharmaceutical Formulations Keywords:

Net analyte signal , pharmaceutical formulation , Simultaneous determination , Standard addition method