The effect of Helicobacter pylori infection on increasing of food allergy induced in animal model

Background and Objective: Helicobacter pylori is the leading cause of bacterial infection with about ۴.۴ billion sufferers worldwide and more than ۱۰% of the world's population suffers from food allergies. Some studies show the protective effect of H. pylori neonatal infection on reducing or absent allergic diseases. Various studies have shown increased antigen uptake following infection with H. pylori, and these infections may lead to increased food allergy in children. The aim of this study was to investigate the relation between food allergy and Helicobacter pylori.Methods: In this experimental study, ۲۰ female Hartley guinea pigs were obtained from Razi Institute of Mashhad. Iran. We used cow's milk (CM) (۱ mg/g) with Chlorine toxin (CT) (۰.۳ µg/g), as an adjuvant to induce food allergy in guinea pigs for ۵ times in day for sequential ۵ weeks. In the sixth week, each pig received two doses of CM (۳۰ mg) within ۳۰ minutes, the control group received only CT in the same pattern. Also, bacteria were inoculated by orogastric into the fasted pigs for ۳ consecutive days. The study groups are as follows (۵ mice in each group): ۱. Phosphate buffered saline (PBS) group (control group, A) ۲. CM with CT group (B) ۳. Contaminated with H. pylori (C) ۴. Co-infection of food allergy (cow's milk) and gastric ulcer (H. pylori) group (D). The changes in level of IgE, IFN-γ, IL-۴, and histamine plasma were detected by the ELISA kit (U-Cy Tech biosciences) with Pathological assessment. Data were analyzed by SPSS version۱۶. One-way ANOVA and student T-test were used to compare data. The significance level in this study was <۰.۰۵. Findings: In this study, total serum IgE level in group D was significantly increased compared to other groups (p <۰.۰۰۱). Results of plasma histamine measurement showed a significant increase in group D compared to group A (p <۰.۰۵). The results indicate a significant increase in the level of IL-۴ in groups B and D (p<۰.۰۵). According to our results, in group D, INF- γ was increased significantly compare to other groups (p<۰.۰۵). Gastric atrophy Intestinal metaplasia demonstrate a significant increase in group C compared to group B as well as group A (P <۰.۰۰۱) also intestinal metaplasia in group D, showed a significant increase compared to group C (P <۰.۰۰۱). The mononuclear cell count did not have any significant difference in groups A and C (p>۰.۰۵). In addition, there was no significant difference between groups C and D (p>۰.۰۵).Conclusion: According to results of this study, we confirmed that H. pylori seems to increase food allergy induction. However, more researches are needed to find out this correlation in human

Keywords:

Helicobacter pylori , food allergy , gastro intestinal tract