G-CSF augments the neuroprotective effect of conditioned medium of dental pulp stem cells against hypoxic neural injury in SH-SY۵Y cells
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-24-12_016
تاریخ نمایه سازی: 20 آذر 1400
Abstract:
Objective(s): Dental pulp stem cells (DPSCs) can differentiate into functional neurons and have the potential for cell therapy in neurological diseases. Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein family shown neuroprotective effect in models of nerve damage.we evaluated the protective effects of G-CSF, conditioned media from DPSCs (DPSCs-CM) and conditioned media from transfected DPSCs with plasmid encoding G-CSF (DPSC-CMT) on SH-SY۵Y exposed to CoCl۲ as a model of hypoxia-induced neural damage.Materials and Methods: SH-SY۵Y exposed to CoCl۲ were treated with DPSCs-CM, G-CSF, simultaneous combination of DPSCs-CM and G-CSF and finally DPSC-CMT. Cell viability and apoptosis were determined by resazurin (or lactate dehydrogenase (LDH) assay alternatively) and propidium iodide (PI) staining. Western blot analysis was performed to detect changes in apoptotic protein levels. The interleukin-۶ and interleukin-۱۰ IL۶/IL۱۰ levels were measured with Enzyme-Linked Immunosorbent Assay (ELISA).Results: DPSCs-CM and G-CSF were able to significantly protect SH-SY۵Y against neural cell damage caused by CoCl۲ according to resazurin and LDH analysis. Also, the percentage of apoptotic cells decreased when SH-SY۵Y were treated with DPSCs-CM and G-CSF simultaneously. After transfection of DPSCs with G-CSF plasmid, DPSC-CMT could significantly improve the protection. The amount of β-catenin, cleaved PARP and caspase-۳ were significantly decreased and the expression of survivin was considerably increased when hypoxic SH-SY۵Y treated with DPSCs-CM plus G-CSF according to Western blot. Decreased level of IL-۶/IL-۱۰, which exposed to CoCl۲, after treatment with DPSCs-CM indicated the suppression of inflammatory mediators.Conclusion: Combination therapy of G-CSF and DPSCs-CM improved the protective activity.
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Authors
Farahnaz Ahmadi
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra salmasi
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Majid mojarad
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Atieh Eslahi
Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Tayarani-Najaran
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
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