Vestigation of explant and plant growth regulators on callogenesis, Indirect shoot Regeneration and shoot rooting in Echinacea Purpurea L

Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu, related ailments and stimulate the immune system. In vitro propagation is a suitable response to increase the demand for this medicinal plant and indirect regeneration is one of the useful methods for plant propagation. The effects of explant (leaf and petiole) and different concentrations ۶-benzylaminopurine(BAP) and Thidiazuron (TDZ) alone or combination with naphthalene acetic acid (NAA) and indole-۳-butyric acid (IBA) on callus induction as well as the effect of explant (leaf and petiole) and ۲mg/l BAP hormone on the indirect shoot regeneration of callus were evaluated in two separated experiments. The effects of IBA or NAA on shoot rooting were investigated. The study were conducted as factorial in a completely randomized design (CRD). The results indicated that highest callogenesis were obtained using different concentrations TDZ and IBA. The highest shooting ( ۹۲/۸۵ %) was obtained from the leaf explant and by use of BAP (۲ mg/l) and NAA (۰.۱ mg/l). high rooting was achieved using MS media supplemented with NAA. Highest number root per explant (۸/۱۶) was achieved using MS media supplemented with ۰/۵ mg/l NAA. The good potential of this species for indirect shoot regeneration was shown in this research can be used in micropropagation programs , gene transferand and transgenic plants production

Keywords:

Callogenesis , Echinacea pupurea , rooting , shooting , regeneration