Protective effect of an isolated polysaccharide from Rosa canina in STZ-treated Rin۵-F cell line through modulation of cell cycle

Polysaccharide isolated from medicinal herbs plays an essential role in treatment of metabolic diseases including diabetes. To understand the underlying molecular mechanism of isolated polysaccharide from Rosa canina, modulation of cell cycle was evaluated in a diabetic model, STZ-treated Rin۵-F cell line. Herein, polysaccharide was extracted from Rosa canina and evaluated by using infrared radiation (IR), high-performance liquid chromatography diode array detector tandem mass spectroscopy (HPLC-DAD MS/MS) and nuclear magnetic chromatography (NMR) systems. Rin-۵F cells were treated with STZ and isolated polysaccharide alone and in combination. The viability of the cells, cell cycle and the expression of Bax, Bcl۲ and cyclin d۱ were analyzed using MTT assay, flow cytometry and qRT-PCR, and western blotting respectively. Polysaccharide increased the viability of Rin۵-F cells exposed upon STZ as post and pre-treatments. Cell cycle analyses indicated the protective effect of isolated polysaccharide through scale down in the sub G۱ phase and scale up in S phase. The ratio of Bax/Bcl۲ was decreased while the expression of cyclin d۱ increased in STZ and polysaccharide co-treated-Rin۵-F cells compared to STZ-treated cells. The results indicated that the protective effects of isolated polysaccharide was mediated by the modulation of cell cycle and it is expected that the results paved the way to speculate novel anti-diabetic strategies.

Keywords:

Polysaccharide , Rin۵-F cells , Cell cycle , Bax , Bcl۲ , cyclin d۱