Determination of essential oil compounds of Achillea milleolifium extracted using different distillation methods

Three different methods: hydro distillation, microwave-assisted hydrodistillation and Ultrasonic assist with headspace solid phase microextraction have been applied for the first time together for the extraction of volatile organic compounds of Achillea millefolium L from Iran. The oils obtained were analyzed by GC–MS. The extraction time while using the MAHD is no more than ۲۴ min using a microwave power of ۳۰۰ W. In the presence of Ultrasonic assist with headspace solid phase microextraction (UA-HS-SPME), the highest extraction efficiency was achieved with a ۱۰۰ μm polyd imethylsiloxane fiber. Different experimental parameters such as fiber's coating type, sonication time, extraction time and temperature, and desorption time were investigated. The major component by three methods of HD, MAHD and UA-HS-SPME were carvacrol as ۶۴.۳۱%, ۵۴.۰۴% and ۷۷.۱۳% respectively. Differences were observed both in the composition of the essential oil and from the energetic point of view

Keywords:

Solid phase microextraction , Achillea millefolium L , microwave hydrodistillation , Gas chromatography–mass spectrometry