Quantitative and Qualitative analysis of Rutin and Quercetin in Lycium barbarum L. plant using HPLC-PDA and determining the amount of Total Phenols and Flavonoids in hydroalcoholic extract

Background: Goji berry is a scientific name (Lycium barbarum L.) from Solanaceae family which has been widely used in traditional medicine.Objective: The main objective of this study was to develop a novel method using HPLC to measure the amount of rutin and quercetin flavonoids. Also, considering the reported medicinal properties of leaf and fruit of Lycium barbarum and its commerciality and reports of significant amounts of phenol and flavonoids in the leaves of the plant, the total content of these compounds in the fruit of the plant were also measured (phenols and flavonoids).Results: The results were reported based on HPLC data for the two rutin and quercetin ۸۴۲ ± ۱.۲ μg/g plant and ۲۲ ± ۱/۳ μg/g plant, respectively.The spectrophotometric method was used to determine the total amount of ۸۰% methanolic extract of phenol and flavonoid extract ۱۲.۹۲ ± ۱.۹۷ mg Gallic acid/g plant and ۳.۷۱ ± ۰.۵۶ mg Rutin /g plant, respectively.Conclusion: According to HPLC data, the presence of flavonoids of rutin and quercetin in the hydroalcoholic extract of the fruit of the plant were reported and their quantities were reported for first time. The new HPLC method used for these two compounds showed its effectiveness in the measurements. Determination of total phenol and flavonoid amounts showed significant presence of these compounds.

Keywords:

Goji berry plant , Hydroalcoholic extract , HPLC , Total Phenols and Flavonoids