Study of electrochemical behavior of orchis plant by cyclic voltammetry and squar wave

Orchis is a genus in the orchid family, occurring mainly in Europe and Northwest Africa, and ranging as far as Tibet, Mongolia, and Xinjiang. The name is from the Ancient Greek ὄρχις orchis, meaning "testicle", from the appearance of the paired subterranean tuberoids. In recent years, more attention has been devoted to herbal medicines. Up to now, many compounds with therapeutic effects has been extracted from the herbs۱-۵. The aim of this study is to evaluate the antioxidant and the antimicrobial effect of Orchis. Also We have investigated atioxidan capacity Orchis using with cyclic voltammetry and squar wave voltammetry methods. Effect of pH and some metal on the antioxidant capacity has been studied (Fe۳+,Ca۲+,Cd۲+,Co۲+and Cr۳+).The kinetic data were extracted from cyclic voltammograms with the help of digital simulation. The homogeneous and heterogeneous rate constants were estimated by comparing the experimental cyclic voltammetric responses with the digital simulated results. We have investigated atioxidan capacity Orchis using with cyclic voltammetry and squar wave voltammetry methods. The kinetic data were extracted from cyclic voltammograms with the help of digital simulation. The homogeneous and heterogeneous rate constants were estimated by comparing the experimental cyclic voltammetric responses with the digital simulated results. The reduction of Orchis is pH independent and occurs at very high potentials, which means that it can be studied only at pH values higher than ۵. The use of buffer electrolyte in a mixed acetonitrile/water solvent proved very convenient for preventing strong adsorption of the analyte on the electrode surface and enabling better repro- ducibility and sensitivity. Adsorptive linear sweep square wave voltammetry permitted accurate quantification of Orchis in commonly used pharmaceutical drugs in the micromolar range after a very simple and rapid sample treatment. Good precision was obtained .This electroanalytical method can be used for determination of thera- peutic doses of Orchis in biological fluids if coupled with high performance liquid chromatog- raphy (HPLC) with electrochemical detection.

Keywords:

Antioxidant , Electrochemical , Cyclic Voltammetry , squar wave voltammetry