Isolation and molecular identification of endophytic fungi from endemic plant Rhabdosciadium aucheri

Endophytes are micro-organisms that inhabit into tissues of plants without any obvious signs. All vascular and non-vascular plants on land and sea are the habitat of endophytic fungi. Presence of endophytic microorganisms in herbaceous and woody tissues of all plant organs including roots, Stem, Branch, Skin, Leaves petiole, Flower, Fruit and seed has been proved. Endophytic flora is varied in different hosts based on population and diversity and it depends on the geographical location of the host. Rhabdosciadium aucheri belongs to the Apiaceae family, an exclusive species of Iran that grows in the provinces of Hamedan, Lorestan, Chaharmahal and Bakhtiari Kohgiluyeh and Boyer-Ahmad and Tehran. The aim of this study was to isolate and molecular identification of endophytic fungi in Rhabdosciadium aucheri, an endemic plant of Hamadan province. The plant was collected in spring, ۲۲ July ۲۰۲۰ from different parts of Hamadan province. After surface disinfection and cutting into smaller pieces, the samples were cultured in PDA medium and examined after coding and initial growth. Identified species include Altrnaria radicina, Altrnaria rosae, Altrnaria sp., Altrnaria carotiincuitae, Penicillum sp., Penicillum sanguifluum, Paraphoma radicina, Neonectria candida, Cadophora sp., Montagnula sp. In this study, the most abundant isolated fungi belong to the genus Altrnaria radicina, which has the highest abundance in the roots of the plant related to Imamzadeh Kuh region. Genus Penicillum sp., Paraphoma radicina and Neonectria candida, which have the highest abundance of endophytic fungi in the roots of Tuyserkan Ski slope, respectively. All species introduced for the first time as endophytic fungi from Rhabdosciadium aucheri, an endemic plant, are reported in Iran and the world. This study is the first study of endophytic fungi from the Rhabdosciadium aucheri in Iran and the world.

Keywords:

Molecular identification , Endophytic fungus , Rhabdosciadium aucheri