Detection to trace aluminum ion of pharmaceutical wastewater using synthesis of Schiff-based chemosensor

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_EHEM-8-4_008

تاریخ نمایه سازی: 28 آذر 1400

Abstract:

Background: The aim of this research was to develop a fluorogenic sensor for Al۳ + ions, which have been identified as a possible food and drinking water pollutant by the WHO and considered to be harmful to human health. Methods: The sensing mechanism was based on excited-state intramolecular proton transfer, with the intramolecular rotation restriction occurring after binding with the analyte. The probe attaches Al۳ + selectively and emits strong emission in ۴:۱ H۲O/MeOH (v/v) solution while irradiated at ۴۰۰ nm in the presence of a wide number of cations, acting as a “turn-on” fluorescence chemosensor. The range of detection for Al۳ + is ۳.۳ nM (۳ method), which is more than ۲۰۰ times more responsive than the WHO suggested limit of ۷.۴ mM (۳σ method). Mass spectra, job plot, and Benesi-Hildebrand plot were used to determine the formation of the ۱:۱ metal-to-ligand complex. Results: Aluminum (Al) ion content in effluent obtained from the pharmaceutical sector is ۰.۳۸۱ mM, which is a trace amount. A separate in vitro experiment indicates that the probe can precisely perceive Al۳ + ions in a cell line. The sensor-based method is developed to detect ۳.۳ nM of Al۳ + ions, which is significantly less than the WHO max. Conclusion: The probe to detect Al۳ + ions in live cells. HL becomes a flexible sensor for recognizing intracellular Al۳ + in human liver cancer cell line Hep G۲ and human lung fibroblast cell lines by fluorescence cell imaging procedures, and the probe’s non-toxicity has been proven by MTT tests up to ۱۰۰M.

Authors

Mengistu Jemberu Dagnaw

Corresponding author:Department of Mechanical Engineering, Institute of Engineering and Technology, Wollega University, Post Box No.۳۹۵, Nekemte, Ethiopia

Mahesh Gopal

Department of Mechanical Engineering, Institute of Engineering and Technology, Wollega University, Post Box No.۳۹۵, Nekemte, Ethiopia

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