Evaluation of isolation method in remaining of differentiation potential of perivascular human umbilicalcord mesenchymal stem cells toward male germ cell-like

Background and aims: Infertility is one of the most common problems among couples. Generation of male germ cells from adult stem cellsis a current promising priority of researchers. This study aimed to investigate the potential of human umbilical cord mesenchymal stem cells(hUMSCs) on the expression of male germ cell markers after isolating by this method.Methods: The hUMSCs was incubated with retinoic acid, testosterone, and conditioned medium (prepared from testicular cell cultures of۷-day-old mice) during ۳ days. The bands were visualized and densitometry was accomplished using LI-COR Biosciences software.Results: The high expression levels of C-KIT, DAZL, PIWIL۲, and DDX۴ in mRNA and protein levels were observed in treated hUMSCs.Conclusion: Results of reverse transcription polymerase chain reaction (RT-PCR) and western blotting showed that method of isolation hadno adverse effects on differentiation potential of hUMSCs.

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۱۳ May ۲۰۲۰ , Accepted: ۱۸ October ۲۰۲۰ , ePublished: ۳۰ June ۲۰۲۱